Production of Bio-Based Isoprene by the Mevalonate Pathway Cassette in Ralstonia eutropha

Application of the Taguchi Design for Production of Poly(β-hydroxybutyrate) by Ralstonia eutropha

The Taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(PHB) production by Ralstonia eutropha. The optimum condition was obtained as: fructose concentration, 15 g/L; C/N ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° C; seed age, 15 h. At optimu...

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

BACKGROUND Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives. RESULTS In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80 mg/L isoprene. Codon optimizatio...

Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E. coli

The depleting petroleum reserve, increasingly severe energy crisis, and global climate change are reigniting enthusiasm for seeking sustainable technologies to replace petroleum as a source of fuel and chemicals. In this paper, the efficiency of the MVA pathway on isoprene production has been improved as follows: firstly, in order to increase MVA production, the source of the "upper pathway" wh...

Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture

Running title: Branched-chain alcohols production in engineered Ralstonia eutropha